GRAMD1C Protein (AA 1-662) (His tag)
custom-made
GRAMD1C
Origin: Human
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- GRAMD1C (GRAM Domain Containing 1C (GRAMD1C))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-662
- Origin
- Human
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GRAMD1C protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant GRAMD1C Protein expressed in mammalian cells.
- Sequence
- MEGAPTVRQV MNEGDSSLAT DLQEDVEENP SPTVEENNVV VKKQGPNLHN WSGDWSFWIS SSTYKDRNEE YRRQFTHLPD TERLIADYAC ALQRDILLQG RLYLSENWLC FYSNIFRWET TISIALKNIT FMTKEKTARL IPNAIQIVTE SEKFFFTSFG ARDRSYLSIF RLWQNVLLDK SLTRQEFWQL LQQNYGTELG LNAEEMENLS LSIEDVQPRS PGRSSLDDSG ERDEKLSKSI SFTSESISRV SETESFDGNS SKGGLGKEES QNEKQTKKSL LPTLEKKLTR VPSKSLDLNK NEYLSLDKSS TSDSVDEENV PEKDLHGRLF INRIFHISAD RMFELLFTSS RFMQKFASSR NIIDVVSTPW TAELGGDQLR TMTYTIVLNS PLTGKCTAAT EKQTLYKESR EARFYLVDSE VLTHDVPYHD YFYTVNRYCI IRSSKQKCRL RVSTDLKYRK QPWGLVKSLI EKNSWSSLED YFKQLESDLL IEESVLNQAI EDPGKLTGLR RRRRTFNRTA ETVPKLSSQH SSGDVGLGAK GDITGKKKEM ENYNVTLIVV MSIFVLLLVL LNVTLFLKLS KIEHAAQSFY RLRLQEEKSL NLASDMVSRA ETIQKNKDQA HRLKGVLRDS IVMLEQLKSS LIMLQKTFDL LNKNKTGMAV ES Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- GRAMD1C (GRAM Domain Containing 1C (GRAMD1C))
- Alternative Name
- GRAMD1C
- Background
- Protein Aster-C (GRAM domain-containing protein 1C),FUNCTION: Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity). {ECO:0000250|UniProtKB:Q8CI52}.
- Molecular Weight
- 76.0 kDa
- UniProt
- Q8IYS0
- AlphaFold
- Q8IYS0
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