Heat Shock Protein 65 (HSP65) antibody
This Mouse Monoclonal antibody specifically detects in EIA and WB. It exhibits reactivity toward Mycobacteria.
Catalog No. ABIN2859572
Quick Overview for Heat Shock Protein 65 (HSP65) antibody (ABIN2859572)
Target
See all Heat Shock Protein 65 (HSP65) products
Heat Shock Protein 65 (HSP65)
Reactivity
Mycobacteria
Host
Mouse
Clonality
Monoclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
Clone
BDI578
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Specificity
- This antibody is reactive with Hsp65 (GroEL) of M. tuberculosis. Does not react with M. bovis, M. avium, M. phlei, M. parafortuitum, Rhodococcus sp., B. subtilis, S. pneumoniae, and E. coli.
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Characteristics
- Synonyms: 60 kDa chaperonin 2, Protein Cpn60-2, groEL protein 2, Cell wall protein A, Antigen A,groL2, groEL-2, groEL2, hsp65, Rv0440, MT0456, MTV037.04
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Purification
- Protein A chromatography
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Purity
- > 90 % pure
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Immunogen
- Purified protein derivative (PPD)
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Isotype
- IgG2a
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Application Notes
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ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0,1 mg/mL (OD280nm, E0.1% = 1.4)
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Buffer
- 0.01 M PBS, pH 7.2 containing 0.09 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
Expiry Date
- 12 months
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- Heat Shock Protein 65 (HSP65)
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Alternative Name
- Heat Shock Protein 65 / HSP65
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Background
- Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure, most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.Synonyms: 60 kDa chaperonin 2, Antigen A, Cell wall protein A, MT0456, MTV037.04, Protein Cpn60-2, Rv0440, groEL protein 2, groEL-2, groEL2, groL2, hsp65
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Gene ID
- 886354
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UniProt
- P0A520
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Pathways
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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