Z-VAD-FMK (General Caspase Inhibitor)
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- Application
- Functional Studies (Func), Cell Culture (CC), Blocking Reagent (BR), Inhibition Assay (InhA)
- Purpose
- A pan-caspase inhibitor
- Brand
- BD Pharmingen™
- Sequence
- Z-Val-Ala-Asp(OMe)-FMK
- Characteristics
- Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-VAD-FMK is a cell permeable general caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and inhibits apoptosis. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. Z-VAD-FMK has a molecular weight of 467 Daltons. Flow cytometric analysis of apoptosis in Jurkat cells (Human T-cell leukemia, ATCC TIB-152). Jurkat cells were preincubated with the following: no inhibitor (upper left and bottom left panels), 20 μM Z-VAD-FMK (upper center and bottom center panels) or 20 μM negative control inhibitor (Z-FA-FMK, upper right and bottom right panels) (Cat. No. 550411) for 30 min, and then either left untreated (bottom row) or treated with 4 μM of campthothecin for 3 hr (top row). Following incubation, cells were collected and stained with PE Annexin V (Cat. No. 559763) to identify cells undergoing apoptosis. The results indicate that in campthothecin treated cells, approximately 42 % of the cells were induced to undergo apoptosis and the use of the general caspase inhibitor Z-VAD-FMK reduced the level of apoptosis to that observed in the untreated control. Cells treated with Z-FA-FMK (Cat. No. 550411) showed similar results to the campthothecin treated cells without inhibitor, indicating that the negative control inhibitor did not attenuate apoptosis.
- Purification
- Purified
- Chemical Name
- Z-VAD fluoromethylketone
- Formula
- C22H30N3O7F
- Permeability
- Cell-permeable
- Solubility
- DMSO
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- Comment
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BD Pharmingen™ Z-VAD-FMK, General Caspase Inhibitor – Purified
Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-VAD-FMK is a cell permeable general caspase inhibitor that irreversibly binds to the catalytic site of caspase proteases and inhibits apoptosis. The peptide is O-methylated in the P1 position on aspartic acid providing enhanced stability and increased cell permeability. Z-VAD-FMK has a molecular weight of 467 Daltons. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the Z-VAD-FMK inhibitor in DMSO before use. The reconstituted Z-VAD-FMK inhibitor may be stored in small aliquots at -20 °C.
- Storage
- -20 °C
- Storage Comment
- Avoid multiple freeze-thaws of product. Store the lyophilized Z-VAD-FMK inhibitor at -20°C. Reconstitute the Z-VAD-FMK inhibitor in DMSO before use. The reconstituted Z-VAD-FMK inhibitor may be stored in small aliquots at -20°C.
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Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors." in: Journal of cellular physiology, Vol. 178, Issue 2, pp. 133-43, (1999) (PubMed).
: "Caspases: enemies within." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).
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Function of caspases in regulating apoptosis caused by erythropoietin deprivation in erythroid progenitors." in: Journal of cellular physiology, Vol. 178, Issue 2, pp. 133-43, (1999) (PubMed).
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- Molecular Weight
- 467 Da
- CAS-No
- 187389-52-2
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