-
- Target See all IgM products
- IgM
-
Host
- Mouse
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Supplier Product No.
- 010-0107
- Supplier
- Rockland
- Purpose
- Mouse IgM Whole Molecule
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (μ chain specific). No reaction was observed against anti-Mouse IgG F(c). Some light chain cross-reactivity will occur with anti-Mouse IgG.
- Characteristics
- Mouse Immunoglobulin M
- Purification
- Mouse IgM was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Isotype
- IgM
-
Mouse IgM isotype control (FITC)
IsoC, FACS, IF IgM FITC
Mouse IgM isotype controlIsoC, FACS IgM unconjugated
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (APC)IsoC, FACS, IF IgM APC
Mouse IgM isotype control (PE-Cy5)IsoC, FACS, IF IgM PE-Cy5
Mouse IgM isotype control (FITC)IsoC, FACS, IF IgM FITC
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype control (PE)IsoC, FACS, IF IgM PE
-
- Application Notes
-
Application Note: Mouse IgM whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.
Western Blot Dilution: user optimized
ELISA Dilution: user optimized
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.16 mg/mL
- Buffer
-
Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
- Background: Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen. IgM is predominantly produced in the spleen. Mouse IgM is formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites.
-
You are here:
