Quick Overview for Mouse IgG2b isotype control (PE) (ABIN1045090)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-0842
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Supplier
- Rockland
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Purpose
- Mouse IgG2b Isotype Control Phycoerythrin Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
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Characteristics
- Phycoerythrin conjugation Mouse IgG2b isotype control, Mouse IgG2b subclass isotype control PE conjugated
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Purification
- This product was prepared from ascitic fluid by affinity chromatography.
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Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b unconjugated
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b FITC
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b APC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b PE
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b PerCP
Mouse IgG2b isotype control (FITC)FACS, ELISA Monoclonal A-1 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
Mouse IgG2b isotype control (iFluor™700)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700
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Application Notes
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Flow Cytometry Dilution: User Optimized
Application Note: Mouse IgG2b isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
IF Microscopy Dilution: 1:1000-1:5000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
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Expiry Date
- 12 months
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- IgG2b
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Target Type
- Antibody
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Background
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
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