Quick Overview for Mouse IgG3 isotype control (PE) (ABIN125735)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Mouse
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Purpose
- Mouse IgG3 Isotype Control PE
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Specificity
- This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.
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No Cross-Reactivity
- Human, Mouse, Pig, Rat
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Cross-Reactivity (Details)
- Other species Not tested (expected negative),Other species Not tested
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Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype control (PE-Cy7)FACS, ELISA Monoclonal B10 IgG3 PE-Cy7
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated
Human IgG3 isotype controlIsoC Polyclonal IgG3 unconjugated
Mouse IgG3 isotype controlFACS, ELISA, IsoC, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PPV-07 IgG3 unconjugated
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Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
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Restrictions
- For Research Use only
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Concentration
- 0.1 mg/mL
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Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
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Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user. -
Storage
- 4 °C
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Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- IgG3
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Target Type
- Antibody
Target
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