(5 references)Quick Overview for HEK-293 Cells IgG1 Isotype Control (ABIN2181271)
Target
Application
Isotype
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Biological Activity
- Active
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Reactivity
- Human
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Host
- HEK-293 Cells
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Characteristics
- This protein carries no "tag". The protein has a calculated MW of 26 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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Purity
- >95 % as determined by SDS-PAGE.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated
Mouse IgG1 isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated
Mouse IgG1 isotype control (Biotin)FACS, ELISA Monoclonal 15H6 IgG1 Biotin
Mouse IgG1 isotype control (APC-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7
Mouse IgG1 isotype control (PE-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Buffer
- 50 mM Tris, 100 mM Glycine, pH 7.5
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C in lyophilized state after receipt. For long term storage, upon reconstitution rhIgG1 Fc should be aliquot and store at -20°C or -80°C.
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: "Impact of Payload Hydrophobicity on the Stability of Antibody-Drug Conjugates." in: Molecular pharmaceutics, Vol. 15, Issue 7, pp. 2656-2664, (2019) (PubMed).
: "Frizzled proteins are colonic epithelial receptors for C. difficile toxin B." in: Nature, Vol. 538, Issue 7625, pp. 350-355, (2017) (PubMed).
: "Single chain Fc-dimer-human growth hormone fusion protein for improved drug delivery." in: Biomaterials, Vol. 117, pp. 24-31, (2017) (PubMed).
: "GR and ER Coactivation Alters the Expression of Differentiation Genes and Associates with Improved ER+ Breast Cancer Outcome." in: Molecular cancer research : MCR, Vol. 14, Issue 8, pp. 707-19, (2017) (PubMed).
: "Inhibition of the angiopoietin/Tie2 axis induces immunogenic modulation, which sensitizes human tumor cells to immune attack." in: Journal for immunotherapy of cancer, Vol. 3, pp. 52, (2015) (PubMed).
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: "Impact of Payload Hydrophobicity on the Stability of Antibody-Drug Conjugates." in: Molecular pharmaceutics, Vol. 15, Issue 7, pp. 2656-2664, (2019) (PubMed).
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- IgG1
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Target Type
- Antibody
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Background
- Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
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Molecular Weight
- 26 kDa
Target
-
