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- Target See all IgG2a products
- IgG2a
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Host
- Mouse
- Conjugate
- Biotin
- Application
- Flow Cytometry (FACS), Isotype Control (IsoC)
- Characteristics
- The G155-178 clone has an unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone G155-178 was selected as an isotype control following screening for low background on a variety of mouse and human tissues. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- TNP-keyhole limpet hemocyanin
- Isotype
- IgG2a
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 20 μL
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
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The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
: "
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The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Target Type
- Antibody
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