(1 reference)Quick Overview for Mouse IgG2a isotype control (Biotin) (ABIN2688783)
Target
Conjugate
Application
Isotype
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Host
- Mouse
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Characteristics
- The G155-178 clone has an unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone G155-178 was selected as an isotype control following screening for low background on a variety of mouse and human tissues. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)
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Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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Immunogen
- TNP-keyhole limpet hemocyanin
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Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa APC
Mouse IgG2a isotype control (PerCP)FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PerCP
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Mouse IgG2a isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated
Rat IgG2a isotype control (FITC)FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC
Mouse IgG2a isotype control (FITC)FACS, IsoC Monoclonal MOPC-173 IgG2a kappa FITC
Mouse IgG2a isotype control (Biotin)FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin
Mouse IgG2a isotype control (PE)FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PE
Mouse IgG2a isotype control (FITC)FACS, ELISA Monoclonal HOPC-1 IgG2a FITC
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 20 μL
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
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: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
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: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
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- IgG2a
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Target Type
- Antibody
Target
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