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Mouse IgG2b isotype control (Biotin)

Name: Mouse IgG2b isotype control (Biotin)
Brand: antibodies-online
SKU: ABIN2749091
Price: 442.03 USD
Availability: InStock

FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin

Example of nonspecific mouse IgG2b (MPC-11) biotin signal on human peripheral blood; surface staining, 16 μg/ml. (Mouse IgG2b isotype control (Biotin))

FACS

3 images

(5 references)

Catalog No. ABIN2749091

Quick Overview for Mouse IgG2b isotype control (Biotin) (ABIN2749091)

Target

IgG2b

Clonality

Monoclonal

Conjugate

Biotin

Application

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

MPC-11

Isotype

IgG2b kappa

Host
113

57

2
Mouse

Purpose

Mouse IgG2b Isotype Control Biotin

Specificity

This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

No Cross-Reactivity

Human, Mouse, Rat

Purification

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen

KLH-coupled trinitrophenol
Mouse IgG2b isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated

Mouse IgG2b isotype control
FACS, ELISA Monoclonal A-1 IgG2b unconjugated

Mouse IgG2b isotype control (FITC)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa FITC

Mouse IgG2b isotype control (PerCP)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa PerCP

Mouse IgG2b isotype control (APC)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa APC

Mouse IgG2b isotype control (PE)
FACS, IsoC Monoclonal MPC-11 IgG2b kappa PE

Mouse IgG2b isotype control (FITC)
FACS, ELISA Monoclonal A-1 IgG2b FITC

Mouse IgG2b isotype control (PE)
FACS, ELISA Monoclonal A-1 IgG2b PE

Mouse IgG2b isotype control (iFluor™700)
GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700

Mouse IgG2b isotype control (iFluor™647)
GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™647

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Dewals, Vanderplasschen et al.: "Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid ..." in: Veterinary research, Vol. 42, Issue 1, pp. 95, (2011) (PubMed).

Liang, Zhou, Li, Wan: "Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells." in: World journal of gastroenterology, Vol. 16, Issue 5, pp. 618-24, (2010) (PubMed).

Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

Im, Tapinos, Chae, Illarionov, Besra, DeVries, Modlin, Sieling, Rambukkana, Porcelli: "Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 8, pp. 5226-35, (2006) (PubMed).

Gupta, Wang, Vinson, Dziarski: "Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14012-20, (1999) (PubMed).
Target

IgG2b

Alternative Name

IgG2b kappa

Target Type

Antibody