Quick Overview for Mouse IgG2b isotype control (APC) (ABIN5608811)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-2642
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Supplier
- Rockland
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Purpose
- Mouse IgG2b Isotype Control Allophycocyanin Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Allophycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
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Characteristics
- allophycocyanin conjugated Mouse Isotype control, APC control, mouse IgG2b subclass isotype control
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Purification
- Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A.
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Sterility
- Sterile filtered
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Labeling Ratio
- 1-2
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Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa unconjugated
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b kappa FITC
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b kappa PerCP
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b kappa APC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b kappa PE
Mouse IgG2b isotype control (FITC)FACS, ELISA Monoclonal A-1 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
Mouse IgG2b isotype control (iFluor™700)GMP Grade FACS, IsoC Monoclonal MPC-11 IgG2b iFluor™700
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Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2b kappa isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
FLISA Dilution: User Optimized
ELISA Dilution: 1:2000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
- Optional[Buffer]: None Preservative: 0.01 % (w/v) Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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Expiry Date
- 12 months
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- IgG2b
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Target Type
- Antibody
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Background
- Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
Target
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