Quick Overview for Human IgA Isotype Control (ABIN5633212)
Target
Application
Isotype
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Host
- Human
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Supplier Product No.
- 009-0106-0001
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Supplier
- Rockland
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Purpose
- Human IgA Whole Molecule
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Cross-Reactivity (Details)
- Human IgA whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
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Characteristics
- Human Immunoglobulin A
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Purification
- Human IgA whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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Mouse IgA isotype control
ELISA, FACS Monoclonal S107 IgA unconjugated
Mouse IgA isotype controlELISA, FACS, IsoC, IHC Monoclonal S107 IgA unconjugated
Human IgA isotype controlIsoC Polyclonal IgA unconjugated
Human IgA isotype control (HRP)ELISA, IsoC, WB, IHC Polyclonal IgA HRP
Mouse IgA isotype control (FITC)ELISA, FACS Monoclonal S107 IgA FITC
Human IgA isotype control (Alkaline Phosphatase (AP))ELISA, IsoC, WB, IHC Polyclonal IgA Alkaline Phosphatase (AP)
Mouse IgA isotype control (PE)ELISA, FACS Monoclonal S107 IgA PE
Mouse IgA isotype control (SPRD)ELISA, FACS Monoclonal S107 IgA SPRD
Mouse IgA isotype control (PE-Cy7)ELISA, FACS Monoclonal S107 IgA PE-Cy7
Mouse IgA isotype control (APC-Cy5.5)ELISA, FACS Monoclonal S107 IgA APC-Cy5.5
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Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Human IgA whole molecule has been tested in SDS-Page and Western blot and can be utilized as a control or standard reagent in SDS, Western Blotting and ELISA experiments.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgA
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Target Type
- Antibody
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Background
- Background: Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75 % of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized.
Target
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