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Rat IgG2a Isotype Control

Name: Rat IgG2a Isotype Control
Brand: antibodies-online
SKU: ABIN7014012
Price: 351.26 USD
Availability: InStock

FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal RTG2A1-1 IgG2a unconjugated

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using rat IgG2a Isotype control (RTG2A1-1) purified antibody (concentration in sample 3 μg/mL, DAR APC). (Rat IgG2a Isotype Control)

FACS

1 image

Catalog No. ABIN7014012

Quick Overview for Rat IgG2a Isotype Control (ABIN7014012)

Target

IgG2a

Clonality

Monoclonal

Application

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RTG2A1-1

Isotype

IgG2a

Host
166

96

3

1

1
Rat

Purpose

Rat IgG2a Isotype Control LOW ENDOTOXIN

Specificity

This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.

Purification

Purified by protein-G affinity chromatography.

Endotoxin Level

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Mouse IgG2a isotype control (APC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa APC

Mouse IgG2a isotype control (PerCP)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PerCP

Mouse IgG2a isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated

Mouse IgG2a isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), Func, IHC (p) Monoclonal MOPC-173 IgG2a kappa unconjugated

Rat IgG2a isotype control (FITC)
FACS, IsoC Monoclonal RTG2A1-1 IgG2a FITC

Mouse IgG2a isotype control (FITC)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa FITC

Mouse IgG2a isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa Biotin

Mouse IgG2a isotype control (PE)
FACS, IsoC Monoclonal MOPC-173 IgG2a kappa PE

Rat IgG2a isotype control (Biotin)
FACS IgG2a Biotin

Rat IgG2a isotype control
FACS, ELISA IgG2a unconjugated

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Target

IgG2a

Target Type

Antibody