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Recombinant Human IgG1 isotype control (Biotin)

MALS verified IsoC, ELISA, WB, IHC, in vivo, IF, in vitro, NC Monoclonal IgG1 Recombinant Antibody Biotin
Catalog No. ABIN7488562
$774.00
Plus shipping costs $50.00
Shipping to: United States
Delivery in 5 to 6 Business Days

Quick Overview for Recombinant Human IgG1 isotype control (Biotin) (ABIN7488562)

Target

IgG1

Antibody Type

Recombinant Antibody

Clonality

  • 303
  • 10
  • 7
  • 2
Monoclonal

Conjugate

Biotin

Application

Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), In vivo Studies (in vivo), Immunofluorescence (IF), In vitro Assay (in vitro), Negative Control (NC)

Grade

MALS verified

Isotype

IgG1
  • Host

    • 233
    • 83
    • 65
    • 14
    • 6
    • 5
    • 5
    • 2
    • 1
    • 1
    Human

    Purpose

    Biotinylated Human IgG1 Kappa Isotype Control (Biotin, mAb, carrier free, MALS verified)

    Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.

    Characteristics

    Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. Biotinylated Human IgG1 Kappa Isotype Control (mAb) was purified from HEK293 cell culture. It is the chemically biotin labeled form of Human IgG1 Kappa Isotype Control (mAb) DNP-M2).
  • Application Notes

    This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Storage

    -20 °C
  • Target

    IgG1

    Target Type

    Antibody

    Background

    A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
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