Quick Overview for Rabbit IgG isotype control (Atto 550) (ABIN7582042)
Target
Clonality
Conjugate
Application
Isotype
-
-
Host
- Rabbit
-
Purpose
- A Rabbit isotype control antibody conjugated to the fluorescent dye ATTO Fluor-550.
-
Cross-Reactivity (Details)
- The IgG fraction was purified from non-immunized rabbits.
-
Characteristics
- Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal. Our Rabbit IgG Isotype Control-ATTO Fluor-550 (ABIN7582042), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-550 fluorophore.
-
Purification
- Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
-
-
Hamster IgG isotype control
ELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlELISA, WB, IHC, SDS IgG unconjugated
Rabbit IgG isotype controlFACS, IHC, IP Polyclonal IgG unconjugated
Mouse IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Rabbit IgG isotype control (PE)FACS, ELISA Polyclonal IgG PE
Rabbit IgG isotype controlFACS, ELISA Polyclonal IgG unconjugated
Goat IgG isotype controlIsoC, ELISA, IHC Polyclonal IgG unconjugated
-
-
Application Notes
-
Antigen preadsorption control: NA
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: The isotype control antibody should be used at the exact same dilution of the experimental antibody
Application Dilutions Western blot wb: NA
-
Comment
-
Negative Control: NA
Blocking Peptide: (ABIN7237091)
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- 50 μL double distilled water (DDW).
-
Concentration
- 1 mg/mL
-
Buffer
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- IgG
-
Target Type
- Antibody
-
Background
- NA,NA
Target
-
