Human IgA isotype control (FITC)
Quick Overview for Human IgA isotype control (FITC) (ABIN964160)
Target
Conjugate
Application
Isotype
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Host
- Human
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Supplier Product No.
- 009-0206
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Supplier
- Rockland
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Purpose
- Human IgA Whole Molecule Fluorescein Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgA and anti-Human Serum. No reaction was observed against anti-Human IgM Fc5μ or anti-Human IgG F(c).
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Characteristics
- Human IgA fluorescein conjugated, Human IgA FITC conjugation
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 4.2
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Application Notes
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Application Note: Human IgA whole molecule Fluorescein has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgA
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Target Type
- Antibody
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Background
- Background: Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75 % of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized.
Target
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