Human IgA isotype control (Biotin)
Quick Overview for Human IgA isotype control (Biotin) (ABIN964162)
Target
Conjugate
Application
Isotype
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Host
- Human
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Supplier Product No.
- 009-0606
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Supplier
- Rockland
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Purpose
- Human IgA Whole Molecule Biotin Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgA (a chain specific) and anti-Human Serum. No reaction was observed against anti-Human IgG F(c) or anti-Human IgM (Fc5μ).
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Characteristics
- Human IgA Whole Molecule Biotin Conjugate
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Application Notes
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Application Note: Human IgA whole molecule Biotin is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgA
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Target Type
- Antibody
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Background
- Background: Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75 % of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized.
Target
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