Mouse IgA Isotype Control
(1 reference)
Quick Overview for Mouse IgA Isotype Control (ABIN964165)
Target
Clonality
Application
Clone
Isotype
-
-
Host
- Mouse
-
Supplier Product No.
- 010-001-341
-
Supplier
- Rockland
-
Purpose
- Mouse IgA Kappa (κ) Isotype Control
-
Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
-
Characteristics
- Mouse IgA istoype control, Mouse IgA subclass istoype control, Mouse IgA Kappa
-
Purification
- Mouse IgA Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
-
Sterility
- Sterile filtered
-
-
Mouse IgA isotype control
ELISA, FACS Monoclonal S107 IgA unconjugated
Mouse IgA isotype controlELISA, IsoC, FACS, IHC Monoclonal S107 IgA unconjugated
Human IgA isotype controlIsoC Polyclonal IgA unconjugated
Human IgA isotype control (HRP)ELISA, IsoC, WB, IHC Polyclonal IgA HRP
Mouse IgA isotype control (FITC)ELISA, FACS Monoclonal S107 IgA FITC
Human IgA isotype control (Alkaline Phosphatase (AP))ELISA, IsoC, WB, IHC Polyclonal IgA Alkaline Phosphatase (AP)
Mouse IgA isotype control (PE)ELISA, FACS Monoclonal S107 IgA PE
Mouse IgA isotype control (SPRD)ELISA, FACS Monoclonal S107 IgA SPRD
Mouse IgA isotype control (PE-Cy7)ELISA, FACS Monoclonal S107 IgA PE-Cy7
Mouse IgA isotype control (APC-Cy5.5)ELISA, FACS Monoclonal S107 IgA APC-Cy5.5
-
-
Application Notes
-
Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgA kappa isotype control has been tested in ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1.0 mg/mL
-
Buffer
-
Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide -
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
-
Expiry Date
- 12 months
-
-
-
: "Activated human mast cells induce LOX-1-specific scavenger receptor expression in human monocyte-derived macrophages." in: PloS one, Vol. 9, Issue 9, pp. e108352, (2015) (PubMed).
-
: "Activated human mast cells induce LOX-1-specific scavenger receptor expression in human monocyte-derived macrophages." in: PloS one, Vol. 9, Issue 9, pp. e108352, (2015) (PubMed).
-
- IgA
-
Target Type
- Antibody
-
Background
- Background: Mouse isotype controls are used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In mouse there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain alpha - IgA, gamma - IgG 1, 2a, 2b, 3 and μ - IgM, light chain kappa and lambda.
Target
-
