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Cow IgG Isotype Control

SDS, DB IgG unconjugated Fc fragment
Rockland
Catalog No. ABIN964172
Supplier Product No.: 001-0103

Quick Overview for Cow IgG Isotype Control (ABIN964172)

Target

IgG

Fragment

Fc fragment

Application

SDS-PAGE (SDS), Dot Blot (DB)

Isotype

IgG
  • Host

    • 135
    • 125
    • 124
    • 84
    • 82
    • 64
    • 57
    • 55
    • 45
    • 42
    • 38
    • 33
    • 33
    • 32
    • 25
    • 18
    • 16
    • 10
    • 8
    • 7
    • 3
    • 2
    Cow

    Supplier Product No.

    001-0103

    Supplier

    Rockland

    Purpose

    Bovine IgG Fc Fragment

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c).  No reaction was observed against anti-Bovine IgG F(ab')2 or anti-Papain.

    Characteristics

    Bovine IgG F(c) Agarose Conjugate, Bovine IgG Fc Agarose, Bovine Fc fragment Agarose conjugate

    Purification

    This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. 

    Sterility

    Sterile filtered
  • Application Notes

    Application Note: Bovine IgG F(c) has been tested in SDS-Page and Dot Blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target

    IgG

    Target Type

    Antibody

    Background

    Background: Bovine IgG F(c) Fragment Agarose Conjugated product is made from IgG, secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins followed by agarose conjugation.
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