Human IgG isotype control (Agarose Beads)
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- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
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Host
- Human
- Conjugate
- Agarose Beads
- Application
- Immunoprecipitation (IP)
- Supplier Product No.
- 009-0051
- Supplier
- Rockland
- Purpose
- Human IgG F(ab')2 Fragment Agarose Conjugated
- Characteristics
- Human IgG F(ab')2 fragment Agarose Conjugate, Human IgG Fab2 Agarose
- Purification
- This product is a purified Human IgG isolated from normal Human Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 mg of anti-Human IgG.
- Isotype
- IgG
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- Application Notes
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Application Note: Human IgG F(ab')2 Agarose is suitable for immunoprecipitation and Immunoaffinity purifications.
Immunoprecipitation Dilution: User Optimized
- Restrictions
- For Research Use only
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- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store vial at 4° C prior to opening. DO NOT FREEZE.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Background: Human IgG F(ab')2 Fragment Agarose Conjugated is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . Human IgG F(ab')2 Molecules lack the Fc portion of Human IgG and therefore receptors that bind Human IgG Fc will not bind Human IgG F(ab')2 Molecules. This product possesses the F(ab')2 fragment, recognized by the two F(ab) fragments yielded from the digestion of the antibody below the disulfide bond hinge region followed by agarose conjugation.
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