Mouse IgG1 isotype control (FITC)
Quick Overview for Mouse IgG1 isotype control (FITC) (ABIN964456)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-0240
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Supplier
- Rockland
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Purpose
- Mouse IgG1 Isotype Control Fluorescein Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG1 was proven by Dot Blot.
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Characteristics
- fluorescein conjugated Mouse IgG1 isotype control, Mouse IgG1 subclass isotype control FITC conjugation
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Purification
- This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorbtion was performed to remove any unwanted subclasses. This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 1.45
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Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 Biotin
Mouse IgG1 isotype control (Cy5)FACS, ELISA Monoclonal 15H6 IgG1 Cy5
Mouse IgG1 isotype control (PE)FACS, ELISA Monoclonal 15H6 IgG1 PE
Mouse IgG1 isotype control (APC)FACS, ELISA Monoclonal 15H6 IgG1 APC
Mouse IgG1 isotype control (SPRD)FACS, ELISA Monoclonal 15H6 IgG1 SPRD
Mouse IgG1 isotype control (APC-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7
Mouse IgG1 isotype control (APC-Cy5.5)FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy5.5
Mouse IgG1 isotype control (PE-Cy7)FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7
Mouse IgG1 isotype control (FITC)FACS, ELISA Monoclonal 15H6 IgG1 FITC
Mouse IgG1 isotype control (FITC)FACS, IsoC, DFA, IFA VI-AP IgG1 FITC
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Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG1 isotype has been tested in ELISA and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG1
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Target Type
- Antibody
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Background
- Background: Isotype control Mouse IgG1 is important for Flow Cytometry. Mouse IgG1 control has no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
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