Mouse IgG2b isotype control (Rhodamine)
Quick Overview for Mouse IgG2b isotype control (Rhodamine) (ABIN964472)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-0042
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Supplier
- Rockland
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Purpose
- Mouse IgG2b Isotype Control Rhodamine Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Rhodamine Conjugated Mouse IgG2b was proven by Dot Blot.
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Characteristics
- Rhodamine conjugated Mouse IgG2b isotype control, Mouse IgG2b subclass isotype control TRITC conjugation
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 2.2
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Mouse IgG2b isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b Biotin
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b unconjugated
Mouse IgG2b isotype control (APC)FACS, IsoC Monoclonal MPC-11 IgG2b APC
Mouse IgG2b isotype control (FITC)FACS, IsoC Monoclonal MPC-11 IgG2b FITC
Mouse IgG2b isotype control (PE)FACS, IsoC Monoclonal MPC-11 IgG2b PE
Mouse IgG2b isotype control (PerCP)FACS, IsoC Monoclonal MPC-11 IgG2b PerCP
Mouse IgG2b isotype controlFACS, ELISA Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype controlFACS, IsoC, ELISA, WB Monoclonal A-1 IgG2b unconjugated
Mouse IgG2b isotype control (Cy3)FACS, ELISA Monoclonal A-1 IgG2b Cy3
Mouse IgG2b isotype control (PE)FACS, ELISA Monoclonal A-1 IgG2b PE
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Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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Concentration
- 0.2 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG2b
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Target Type
- Antibody
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Background
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
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