Mouse IgG3 isotype control (Rhodamine)
Quick Overview for Mouse IgG3 isotype control (Rhodamine) (ABIN964478)
Target
Clonality
Conjugate
Application
Clone
Isotype
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Host
- Mouse
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Supplier Product No.
- 010-0043
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Supplier
- Rockland
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Purpose
- Mouse IgG3 Isotype Control Rhodamine Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Rhodamine Conjugated Mouse IgG3 was proven by Dot Blot.
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Characteristics
- Rhodamine conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control TRITC conjugation
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Labeling Ratio
- 2.6
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Mouse IgG3 isotype control
FACS, IsoC, ELISA, IHC Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (APC)FACS, ELISA Monoclonal B10 IgG3 APC
Mouse IgG3 isotype control (PE)FACS, ELISA Monoclonal B10 IgG3 PE
Mouse IgG3 isotype control (SPRD)FACS, ELISA Monoclonal B10 IgG3 SPRD
Mouse IgG3 isotype control (FITC)FACS, ELISA Monoclonal B10 IgG3 FITC
Mouse IgG3 isotype control (Biotin)FACS, ELISA Monoclonal B10 IgG3 Biotin
Mouse IgG3 isotype controlFACS, ELISA Monoclonal B10 IgG3 unconjugated
Mouse IgG3 isotype control (FITC)IsoC, IF IgG3 FITC
Mouse IgG3 isotype controlFACS, IsoC, ELISA, WB, ICC, IP, IHC (fro), IHC (p) Monoclonal PPV-07 IgG3 unconjugated
Mouse IgG3 isotype control (PE)FACS, IsoC Monoclonal PPV-07 IgG3 PE
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Application Notes
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Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG3 isotype control can be utilized as a control or standard reagent in Flow cytometry, Immunofluorescence, FLISA, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL
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Concentration
- 0.2 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgG3
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Target Type
- Antibody
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Background
- Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
Target
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