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Mouse IgG3 isotype control (FITC)

Name: Mouse IgG3 isotype control (FITC)
SKU: ABIN964480
Price: 129 USD
Availability: InStock

FACS, ELISA, WB, FLISA, FM, DB Monoclonal MG3 IgG3 FITC

Image no. 1 for Mouse IgG3 isotype control (FITC) (ABIN964480) (Mouse IgG3 isotype control (FITC))

1 image

Catalog No. ABIN964480

Supplier Product No.: 010-0243

Quick Overview for Mouse IgG3 isotype control (FITC) (ABIN964480)

Target

IgG3

Clonality

Monoclonal

Conjugate

FITC

Application

Flow Cytometry (FACS), ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Clone

MG3

Isotype

IgG3

Host
58

6

6

1
Mouse

Supplier Product No.

010-0243

Supplier

Rockland

Purpose

Mouse IgG3 Isotype Control Fluorescein Conjugated

Cross-Reactivity (Details)

Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.

Characteristics

Fluorescein conjugated Mouse IgG3 isotype control, Mouse IgG3 subclass isotype control FITC conjugation

Purification

This product has been prepared from serum-free tissue culture supernate by protein A chromatography.

Labeling Ratio

3.3
Mouse IgG3 isotype control (PE)
FACS, ELISA Monoclonal B10 IgG3 PE

Mouse IgG3 isotype control (FITC)
FACS, ELISA Monoclonal B10 IgG3 FITC

Mouse IgG3 isotype control (SPRD)
FACS, ELISA Monoclonal B10 IgG3 SPRD

Mouse IgG3 isotype control (APC)
FACS, ELISA Monoclonal B10 IgG3 APC

Mouse IgG3 isotype control (Biotin)
FACS, ELISA Monoclonal B10 IgG3 Biotin

Mouse IgG3 isotype control (PE-Cy7)
FACS, ELISA Monoclonal B10 IgG3 PE-Cy7

Mouse IgG3 isotype control
FACS, ELISA Monoclonal B10 IgG3 unconjugated

Mouse IgG3 isotype control
FACS, ELISA, IsoC, IHC Monoclonal B10 IgG3 unconjugated

Human IgG3 isotype control
IsoC Polyclonal IgG3 unconjugated

Mouse IgG3 isotype control
FACS, ELISA, IsoC, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PPV-07 IgG3 unconjugated

Application Notes

Flow Cytometry Dilution: 1:1000-1:5000
Application Note: Mouse IgG3 isotype has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
ELISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
Other: User Optimized

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 100 μL

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date

12 months
Target

IgG3

Target Type

Antibody

Background

Background: Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.