Quick Overview for Sheep IgM Isotype Control (ABIN964496)
Target
Application
Isotype
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Host
- Sheep
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Supplier Product No.
- 013-0107
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Supplier
- Rockland
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Purpose
- Sheep IgM Whole Molecule
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Cross-Reactivity (Details)
- Sheep IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and anti-Sheep IgM (μ chain specific). No reaction was observed against anti-Sheep IgG F(c). Some light chain cross reactivity will occur with anti-Sheep IgG.
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Characteristics
- Sheep Immunoglobulin M
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Purification
- Sheep IgM whole molecule was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC
Rat IgM isotype controlFACS, ELISA Monoclonal NIP-M-2 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Rabbit IgM isotype controlIsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated
Rat IgM isotype control (PE)FACS, ELISA Monoclonal NIP-M-2 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (Biotin)FACS, ELISA Monoclonal 11E10 IgM Biotin
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (PE-Cy7)FACS, ELISA Monoclonal 11E10 IgM PE-Cy7
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Application Notes
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Immunohistochemistry Dilution: User Optimized
Application Note: Sheep IgM whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Sheep IgM whole molecule is stable at 4° C prior to restoration. It is recommended to aliquot restored Sheep IgM whole molecule after adding equal parts glycerol for storage at -20° C for extended storage and to prevent repeated freeze-thaw cycles.
Western Blot Dilution: User optimized
Immunoprecipitation Dilution: User optimized
ELISA Dilution: User optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
- Optional[Buffer]: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0 Preservative: 0.1 % (w/v) Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- IgM
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Target Type
- Antibody
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Background
- Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.
Target
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