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Human IgM isotype control (FITC)

DB IgM FITC

Dot Blot Results of Human IgM Whole Molecule Fluorescein Conjugated. (Human IgM isotype control (FITC))

1 image

Catalog No. ABIN964505

Supplier Product No.: 009-0207

$145.00

Plus shipping costs $50.00

1 mg

Shipping to: United States

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Quick Overview for Human IgM isotype control (FITC) (ABIN964505)

Target

IgM

Conjugate

FITC

Application

Dot Blot (DB)

Isotype

IgM

Host
61

53

37

5

2

2

2

2

2

2

2

2

2

2

1
Human

Supplier Product No.

009-0207

Supplier

Rockland

Purpose

Human IgM (myeloma) Whole Molecule Fluorescein Conjugated

Cross-Reactivity (Details)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgM and anti-Human Serum.

Characteristics

Human IgM fluorescein conjugated, Human IgM FITC conjugation

Purification

This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by extensive dialysis against the buffer stated above.

Labeling Ratio

3.98
Mouse IgM isotype control (FITC)
FACS, ELISA Monoclonal 11E10 IgM FITC

Rat IgM isotype control
FACS, ELISA Monoclonal NIP-M-2 IgM unconjugated

Mouse IgM isotype control (PE)
FACS, ELISA Monoclonal 11E10 IgM PE

Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated

Mouse IgM isotype control
IsoC, FACS, ELISA, WB, IHC (fro), IHC (p), ICC, IP Monoclonal PFR-03 IgM unconjugated

Rat IgM isotype control (PE)
FACS, ELISA Monoclonal NIP-M-2 IgM PE

Mouse IgM isotype control (APC)
FACS, ELISA Monoclonal 11E10 IgM APC

Mouse IgM isotype control (Biotin)
FACS, ELISA Monoclonal 11E10 IgM Biotin

Mouse IgM isotype control (SPRD)
FACS, ELISA Monoclonal 11E10 IgM SPRD

Mouse IgM isotype control (PE-Cy7)
FACS, ELISA Monoclonal 11E10 IgM PE-Cy7

Application Notes

Application Note: Human IgM whole molecule Fluorescein conjugation has been tested in dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date

12 months
Target

IgM

Target Type

Antibody

Background

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.

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