Human IgM isotype control (FITC)
Quick Overview for Human IgM isotype control (FITC) (ABIN964506)
Target
Fragment
Conjugate
Application
Isotype
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Host
- Human
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Supplier Product No.
- 009-0209
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Supplier
- Rockland
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Purpose
- Human IgM (myeloma) Fab μ Fragment Fluorescein Conjugated
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgM, anti-Human IgG F(ab')2 and anti-Human Serum. No reaction was observed against anti-Human IgG F(c).
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Characteristics
- Human IgM Fab μ fluorescein conjugation, Human myeloma IgM Fabμ FITC conjugation
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Purification
- This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by enzyme digestion and extensive dialysis against the buffer stated above.
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Labeling Ratio
- 3.5
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Rabbit IgM isotype control
IsoC, ELISA, IHC Polyclonal IgM unconjugated
Mouse IgM isotype controlIsoC, FACS, ELISA, IHC Monoclonal 11E10 IgM unconjugated
Mouse IgM isotype control (PE)FACS, ELISA Monoclonal 11E10 IgM PE
Mouse IgM isotype control (APC)FACS, ELISA Monoclonal 11E10 IgM APC
Mouse IgM isotype control (PE-Cy7)FACS, ELISA Monoclonal 11E10 IgM PE-Cy7
Mouse IgM isotype control (SPRD)FACS, ELISA Monoclonal 11E10 IgM SPRD
Mouse IgM isotype control (FITC)FACS, ELISA Monoclonal 11E10 IgM FITC
Mouse IgM isotype control (FITC)IsoC, FACS Monoclonal PFR-03 IgM FITC
Mouse IgM isotype controlIsoC, FACS, ELISA, WB, IHC (p), IHC (fro), IP, ICC Monoclonal PFR-03 IgM unconjugated
Rat IgM isotype control (APC)FACS, ELISA Monoclonal NIP-M-2 IgM APC
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Application Notes
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Application Note: Human IgM Fab μ fragment Fluorescein conjugation is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Buffer: Restore with deionized water (or equivalent)
Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Expiry Date
- 12 months
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- IgM
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Target Type
- Antibody
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Background
- Background: Human IgM Fab mu Fluorescein is ideal for investigators involved in serum protein component research. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition, alpha and gamma contain approximately 450 amino acids, while μ and ε have approximately 550 amino acids.
Target
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