Choline Assay Kit

Catalog No. ABIN1000299

Quick Overview for Choline Assay Kit (ABIN1000299)

Target: Choline

Application: Biochemical Assay (BCA)

Sample Type: Cell Culture Supernatant, Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine

Product Details

Characteristics: Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline.

Components: Assay Buffer: 10 mL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM Choline.

Material not included: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Application Details

Application Notes: Assays: choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture.
Drug Discovery/Pharmacology: effects of drugs on choline metabolism.

Calculation of Results:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.
Conversions: 1 mM choline equals 10.4 mg/dL, 0.010% or 104 ppm.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

Strilakou, Tsakiris, Kalafatakis, Stylianaki, Karkalousos, Koulouris, Mourouzis, Liapi: "Carnitine modulates crucial myocardial adenosine triphosphatases and acetylcholinesterase enzyme activities in choline-deprived rats." in: Canadian journal of physiology and pharmacology, Vol. 92, Issue 1, pp. 78-84, (2014) (PubMed).

Target Details

Target: Choline

Background: Quantitative determination of choline by colorimetric (570nm) or fluorometric (530nm/590nm) methods.
Procedure: 30 min.

Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety. This method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.