Phospholipd Assay Kit

Catalog No. ABIN1000327

Quick Overview for Phospholipd Assay Kit (ABIN1000327)

Target: Phospholipid

Application: Biochemical Assay (BCA)

Sample Type: Serum, Plasma (no EDTA)

Product Details

Characteristics: Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 - 200 µM, fluorimetric assay 0.6 - 20 µM phospholipid.

Components: Assay Buffer: 10 mL. PLD Enzyme: 120 µL. Enzyme Mix: 120 µL. Dye Reagent: 120 µL. Standard: 400 µL 2 mM phosphatidylcholine.

Material not included: Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader, black flat-bottom uncoated 96-well plates, fluorescence plate reader.

Application Details

Application Notes: Assays: phospholipid in biological samples such as serum and non- EDTA plasma. Drug Discovery/Pharmacology: effects of drugs on choline-containing phospholipid metabolism.

Calculation of Results:

Subtract blank value (#4) from the standard values and plot the OD or F against standard concentrations.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

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Soga, Kinosita, Yoshida, Suzuki: "Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis." in: The Journal of biological chemistry, Vol. 287, Issue 12, pp. 9633-9, (2012) (PubMed).

Haynes, Filippov, Filippova, Yang, Zhang, Duerksen-Hughes: "DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells." in: Biochimica et biophysica acta, Vol. 1821, Issue 7, pp. 943-53, (2012) (PubMed).

Mukherjee, Wu, Barbour, Fang: "Lysophosphatidic acid activates lipogenic pathways and de novo lipid synthesis in ovarian cancer cells." in: The Journal of biological chemistry, Vol. 287, Issue 30, pp. 24990-5000, (2012) (PubMed).

Kim, Steelman, Zhang, Kinney, Li: "Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury." in: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 1, pp. 41-57, (2011) (PubMed).

Stoll, Stadnick, Kollas, Holovati, Glasmacher, Acker, Wolkers: "Liposomes alter thermal phase behavior and composition of red blood cell membranes." in: Biochimica et biophysica acta, Vol. 1808, Issue 1, pp. 474-81, (2010) (PubMed).

Target Details

Target: Phospholipid

Alternative Name: Phospholipd

Background: Quantitative determination of phospholipid by colorimetric (570nm) or fluorimetric (530/585nm) methods.
Procedure: 20 min.

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.