Hexanoyl-Lysine Adduct (HEL) ELISA Kit

Catalog No. ABIN1020379

Product Details

Target: Hexanoyl-Lysine Adduct (HEL)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 2-700 nM/L

Minimum Detection Limit: 2 nM/L

Application: ELISA

Purpose: ELISA kit has been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum.

Sample Type: Cell Culture Samples, Serum, Urine

Analytical Method: Quantitative

Specificity: Specific to N-epsilon-Hexanoyl-Lysine adduct.

Characteristics: Assay Time: Overnight and 2 hours

Components: HEL-coated Microtiter Plate: 1 plate (96 wells)
Primary Antibody (ready to use): 1 vial
Secondary Antibody: 1 vial
Secondary Antibody Buffer: 1 vial
Chromogen (TMBZ solution): 1 vial
Chromogen Buffer: 1 vial
Washing Buffer (5X): 1 vial
Stop Solution: 1 vial
Standard solution (6 levels): 1 vial each
Plate seal: 2 sheets

Material not included: 50 µL micropipettor and pipette tips 8-channel (50-200 µL) micropipettor and tips 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. 4-7°C incubator Microtiter plate reader (measuring wavelength 450 nm)

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Sugiyama, Sun, Nishinohara, Fujita, Masuda, Ochi, Takeuchi: "Expressions of lipid oxidation markers, N(ε)-hexanoyl lysine and acrolein in cisplatin-induced nephrotoxicity in rats." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 73, Issue 6, pp. 821-6, (2011) (PubMed).

Wakamatsu, Dogru, Ayako, Takano, Matsumoto, Ibrahim, Okada, Satake, Fukagawa, Shimazaki, Tsubota, Fujishima: "Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease." in: Molecular vision, Vol. 16, pp. 2465-75, (2010) (PubMed).

Shimizu, Ogawa, Akiyama, Muroi, Yoshizaki, Iwata, Komura, Bae, Sato: "Increased serum levels of N(epsilon)-(hexanoyl)lysine, a new marker of oxidative stress, in systemic sclerosis." in: The Journal of rheumatology, Vol. 35, Issue 11, pp. 2214-9, (2008) (PubMed).

Suzuki, Kazui, Yamamoto, Washiyama, Ohkura, Ohishi, Bashar, Yamashita, Terada, Suzuki, Akuzawa, Fujie: "Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction." in: The Journal of thoracic and cardiovascular surgery, Vol. 133, Issue 3, pp. 710-6, (2007) (PubMed).

Naito, Wu, Nomura, Kodama, Kato, Kato, Osawa: "The protective effects of tetrahydrocurcumin on oxidative stress in cholesterol-fed rabbits." in: Journal of atherosclerosis and thrombosis, Vol. 9, Issue 5, pp. 243-50, (2002) (PubMed).

Ueno, Horio, Uchida, Naito, Nomura, Kato, Tsuda, Toyokuni, Osawa: "Increase in oxidative stress in kidneys of diabetic Akita mice." in: Bioscience, biotechnology, and biochemistry, Vol. 66, Issue 4, pp. 869-72, (2002) (PubMed).

Target Details

Target: Hexanoyl-Lysine Adduct (HEL)

Background: Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.