Quick Overview for IgG ELISA Kit (ABIN1113184)
Target
See all IgG ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 16 ng/mL
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Purpose
- Rapid quantification of IgG in cell culture supernatant. Screening of higher producing clones, follow-up of production process, Pharmacokinetic studies
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Brand
- FastELISA®
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Analytical Method
- Quantitative
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Specificity
- The method enables the detection of natural human IgG as well as recombinant humanized or fully human monoclonal antibodies. Chimeric (human-mouse) recombinant antibodies are detected with the method
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Cross-Reactivity (Details)
- < 1% for the following IgG: Mouse, Cow, Horse, Sheep and Swine and are < 2 % for Rabbit IgG.
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Sensitivity
- 4 ng/mL
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Characteristics
- The human immunoglobulin quantification kit provides a rapid and easy method (one step ELISA) for the quantitative determination of human IgG in cell culture supernatant and serum.
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IgG ELISA Kit
Reactivity: Rabbit Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivity: Rabbit
Mouse TrueBlot® Set (with IP beads)WB, IP Reactivity: Mouse
IgG ELISA KitReactivity: Mouse Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Human Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Chicken Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Rat Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivity: Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Comment
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One Step ELISA 30 min total time
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Assay Time
- < 1 h
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Plate
- Pre-coated
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Protocol
- A polyclonal antibody specific to the gamma heavy chain of human IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and human IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-human IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of human IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Reagent Preparation
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dilution
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Expiry Date
- 12 months
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- IgG
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Alternative Name
- Immunoglobulin G
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Target Type
- Antibody
Target See all IgG ELISA Kits
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