Anti-Brucella IgM Antibody ELISA Kit

Catalog No. ABIN1326798

The Human Anti-Brucella IgM Antibody ELISA Kit (ABIN1326798) is a Colorimetric ELISA Kit designed to quantify Human Anti-Brucella IgM Antibody.

Quick Overview for Anti-Brucella IgM Antibody ELISA Kit (ABIN1326798)

Target: Anti-Brucella IgM Antibody

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Serum

Product Details

Purpose: Diluted patient serum (serum diluent contains sorbent to remove Rheumatoid Factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample

Analytical Method: Qualitative

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Anti-Brucella IgM Antibody

Alternative Name: Brucella (IgM)

Target Type: Antibody, Antibody

Background: Brucella is a gram negative coccobacilli capable of infecting a wide range of animal and man. Of the three species causing human infection, iB. melitensisi is the most patogenicfollowed by iB. suisi and iB. abortusi . Brucellosis is transmitted through contaminated and untreated milk and milk products and by direct contact with infected animals (cattle, sheep, goats, pigs, camels, buffaloes,and, very recently, seals), animal carcasses, and abortion materials. Worldwide, millions of inidual are at risk, especially in developing countries where the infection in animals has not been brought under control, heat treatment procedures of milk (e.g. pasteurization) are not routinely applied, and food habits such as consumption of raw milk.The incubation period of brucellosis is usually one to three weeks, but sometimes may be several months. The illness may be mild and self-limiting or severe. The disease is accompanied by continued, intermittent, or irregular fever, headache, weight loss and generalized aching and fatigue. Urogenital symptoms may dominate the clinical presentation in some patients. This method uses iB. abortus iouter membrane, which is shared by the other species. Brucella IgG and IgA antibodies persist for many years after infection. A significant increase in Brucella IgG level is in patients with symptoms of brucellosis is indicative of recent exposure. IgM antibodies are present in acute brucellosis and also found in about 33% of patients with chronic brucellosis.