Decorin ELISA Kit

Catalog No. ABIN1672790

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Decorin.

Quick Overview for Decorin ELISA Kit (ABIN1672790)

Target: Decorin (DCN)

Binding Specificity: AA 31-359

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details Decorin ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Decorin

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: D31-K359

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D31-K359

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Plate: Pre-coated

Protocol: human Decorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Decorin has been precoated onto 96-well plates. Standards(NSO, D31-K359) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Decorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Decorin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Decorin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Decorin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 128, Standard deviation: 5.76, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 645, Standard deviation: 41.28, CV(%): 6.4
• Sample 3: n=16, Mean(pg/ml): 1274, Standard deviation: 72.62, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 139, Standard deviation: 8.48, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 677, Standard deviation: 56.9, CV(%): 8.4
• Sample 3: n=24, Mean(pg/ml): 1333, Standard deviation: 89.31, CV(%): 6.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN

Background:

Protein Function: May affect the rate of fibrils formation.

Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons(kD) in size.It belongs to the small leucine-rich proteoglycan(SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan(GAG) chain consisting of either chondroitin sulfate(CS) or dermatan sulfate(DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Synonyms: Decorin,Bone proteoglycan II,PG-S2,PG40,DCN,SLRR1B,

Full Gene Name: Decorin

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 1634

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process