SERPINA4 ELISA Kit

Catalog No. ABIN1672812

This Human SERPINA4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human SERPINA4.

Quick Overview for SERPINA4 ELISA Kit (ABIN1672812)

Target: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Binding Specificity: AA 21-427

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details SERPINA4 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallistatin/Serpina4

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Q21-P427

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Q21-P427

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the serpin family.

Tissue Specificity: Expressed by the liver and secreted in plasma.

Plate: Pre-coated

Protocol: human Kallistatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallistatin has been precoated onto 96-well plates. Standards(NSO, Q21-P427) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallistatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallistatin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Kallistatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallistatin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1521, Standard deviation: 77.6, CV(%): 5.1
• Sample 2: n=16, Mean(pg/ml): 3786, Standard deviation: 170.4, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 6866, Standard deviation: 425.7, CV(%): 6.2,
• Sample 1: n=24, Mean(pg/ml): 1637, Standard deviation: 104.8, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 4242, Standard deviation: 237.6, CV(%): 5.6
• Sample 3: n=24, Mean(pg/ml): 7128, Standard deviation: 499, CV(%): 7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for SERPINA4

Target: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Alternative Name: SERPINA4

Background:

Protein Function: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .

Background: Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Synonyms: Kallistatin,Kallikrein inhibitor,Peptidase inhibitor 4,PI-4,Serpin A4,SERPINA4,KST, PI4,

Full Gene Name: Kallistatin

Cellular Localisation: Secreted.

Gene ID: 5267

UniProt: P29622