SERPINA4 ELISA Kit

Catalog No. ABIN6720111

Product Details SERPINA4 ELISA Kit

Target: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: KAIN_HUMAN, Kallikrein inhibitor, Kallistatin, Peptidase inhibitor 4, PI-4, PI4, Serpin A4, SERPINA4

Background: Serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor(serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Gene Name: SERPINA4

Production: Natural and recombinant human Kallistatin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q21-P427

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Kallistatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Kallistatin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for SERPINA4

Target: SERPINA4 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 4 (SERPINA4))

Alternative Name: Kallistatin - Serpina4 (SERPINA4 Products)

Synonyms: KLKR ELISA Kit, Klk6 ELISA Kit, hK1 ELISA Kit, KAL ELISA Kit, KLST ELISA Kit, KST ELISA Kit, PI-4 ELISA Kit, PI4 ELISA Kit, kallistatin ELISA Kit, SERPINA4 ELISA Kit, DKFZp469E1737 ELISA Kit, KALps ELISA Kit, Klst ELISA Kit, kallikrein 1 ELISA Kit, serpin family A member 4 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade A, member 4, pseudogene 1 ELISA Kit, KLK1 ELISA Kit, SERPINA4 ELISA Kit, Serpina4 ELISA Kit, SPIA4 ELISA Kit, Serpina4-ps1 ELISA Kit

Gene ID: 3815

NCBI Accession: NP_000213

UniProt: P10721