CCL4 ELISA Kit

Catalog No. ABIN1672852

Rat CCL4 ELISA Kit Colorimetric assay for quantification of Rat CCL4.

Quick Overview for CCL4 ELISA Kit (ABIN1672852)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Binding Specificity: AA 24-92

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Product Details CCL4 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CCL4/MIP-1 beta

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.Coli
Immunogen sequence: A24-N92

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.Coli
Immunogen sequence: A24-N92

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.Coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CCL4 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CCL4 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 35, Standard deviation: 1.3, CV(%): 3.7
• Sample 2: n=16, Mean(pg/ml): 112, Standard deviation: 6.1, CV(%): 5.4
• Sample 3: n=16, Mean(pg/ml): 367, Standard deviation: 21.3, CV(%): 5.8,
• Sample 1: n=24, Mean(pg/ml): 36, Standard deviation: 1.9, CV(%): 5.3
• Sample 2: n=24, Mean(pg/ml): 116, Standard deviation: 7.4, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 371, Standard deviation: 26.7, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4

Background:

Protein Function: Monokine with inflammatory and chemokinetic properties.

Background: Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14).

Synonyms: C-C motif chemokine 4,Macrophage inflammatory protein 1-beta,MIP-1-beta,Small-inducible cytokine A4,Ccl4,Mip1b, Scya4,

Full Gene Name: C-C motif chemokine 4

Cellular Localisation: Secreted.

Gene ID: 116637

UniProt: P50230