IGFBP7 ELISA Kit

Catalog No. ABIN1672887

This Human IGFBP7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IGFBP7. This ELISA Kit has been cited in1 publication.

Quick Overview for IGFBP7 ELISA Kit (ABIN1672887)

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Binding Specificity: AA 30-282

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625-40000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Product Details IGFBP7 ELISA Kit

Minimum Detection Limit: 625 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP7

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: D30-L282

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <20pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: D30-L282

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Plate: Pre-coated

Protocol: human IGFBP7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP7 has been precoated onto 96-well plates. Standards(NSO, D30-L282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP7 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human IGFBP7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP7 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.101, CV(%): 4.4
• Sample 2: n=16, Mean(ng/ml): 16.2, Standard deviation: 0.729, CV(%): 4.5
• Sample 3: n=16, Mean(ng/ml): 28.1, Standard deviation: 1.43, CV(%): 5.1,
• Sample 1: n=24, Mean(ng/ml): 2.8, Standard deviation: 0.188, CV(%): 6.7
• Sample 2: n=24, Mean(ng/ml): 15.4, Standard deviation: 1.063, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 29.7, Standard deviation: 2.14, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for IGFBP7 ELISA Kit (ABIN1672887)

Benassi, Pazzaglia, Novello, Quattrini, Pollino, Magagnoli, Picci, Conti: "Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma." in: American journal of cancer research, Vol. 5, Issue 11, pp. 3446-54, (2016) (PubMed).

Target Details for IGFBP7

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Alternative Name: IGFBP7

Background:

Protein Function: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. .

Background: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors(IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Synonyms: Insulin-like growth factor-binding protein 7,IBP-7,IGF-binding protein 7,IGFBP-7,IGFBP-rP1,MAC25 protein,PGI2-stimulating factor,Prostacyclin-stimulating factor,Tumor-derived adhesion factor,TAF,IGFBP7,MAC25, PSF,

Full Gene Name: Insulin-like growth factor-binding protein 7

Cellular Localisation: Secreted.

Gene ID: 3490

UniProt: Q16270

Pathways: Growth Factor Binding