SensoLyte® 520 ADAM10 Activity Assay Kit

Catalog No. ABIN1882390

Quick Overview for SensoLyte® 520 ADAM10 Activity Assay Kit (ABIN1882390)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 ADAM10 Activity Assay Kit is an optimized assay that can be used to detect the ADAM 10 activity. The unique FRET substrate contained in the kit was designed to reduce the cross reactivity with ADAM17 (also called tumor necrosis factor-alpha-converting enzyme, TACE). When active ADAM10 cleaves the FRET substrate, it results in an increase of 5-FAM fluorescence monitored at excitation/emission = 490/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.25 ng/mL active ADAM10.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C except component C, which should be stored at -80 °C. Component D can be stored at RT for convenience.

Target Details

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: ADAM10

Background: ADAM10 has been shown to be an effective alpha-secretase in vitro and in vivo. This enzyme acts as a sheddase to cleave cell surface proteins involved in neuropathology, inflammatory response and tumor progression.