SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit

Catalog No. ABIN1882397

Quick Overview for SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit (ABIN1882397)

Target: Alkaline Phosphatase (ALP)

Detection Method: Colorimetric

Product Details

Brand: SensoLyte®

Characteristics: SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Placental alkaline phosphatase standard,

Application Details

Comment:

Reporter Gene Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Keep Component A away from light.

Storage: 4 °C

Storage Comment: Store component B and E at -20 °C. Store other kit components at 4 °C.

References

Cacciatore, Leonard, Chasin: "The isolation of CHO cells with a site conferring a high and reproducible transgene amplification rate." in: Journal of biotechnology, Vol. 164, Issue 2, pp. 346-53, (2013) (PubMed).

Target Details

Target: Alkaline Phosphatase (ALP)

Alternative Name: Alkaline Phosphatase

Background: The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient for kinetic analysis of gene expression over a period of time.