SensoLyte® ONPG b-Galactosidase Assay Kit

Catalog No. ABIN1882416

Quick Overview for SensoLyte® ONPG b-Galactosidase Assay Kit (ABIN1882416)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Detection Method: Colorimetric

Application: Enzyme Activity Assay (EAA)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® ONPG beta-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl beta-D-galactopyranoside (ONPG) to determine the activity of beta-galactosidase in transfected cells or tissues. In the presence of beta-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to beta-galactosidase activity.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Handling Advice: Keep component A away from light.

Storage: -20 °C

Storage Comment: Store all components at -20 °C. Component D can be stored at room temperature for convenience.

Target Details

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: beta-Galactosidase

Target Type: Tag

Background: Beta-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells.