Quick Overview for SensoLyte® 520 Cathepsin K Assay Kit (ABIN1882445)
Target
See all Cathepsin K (CTSK) KitsDetection Method
Application
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Brand
- SensoLyte®
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Characteristics
- The SensoLyte® 520 Cathepsin K Activity Assay Kit uses a QXL™ 520/HiLyte Fluor™ 488 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte Fluor™ 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte Fluor™ 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin K enzyme in biological samples and purified enzyme preparations.
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Cathepsin K (CTSK) ELISA Kit
CTSK Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin K (CTSK) ELISA KitCTSK Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin K (CTSK) ELISA KitCTSK Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cathepsin K (CTSK) ELISA KitCTSK Reactivity: Rabbit Colorimetric Sandwich ELISA 1.563 pmol/L - 100 pmol/L Plasma, Serum, Tissue Homogenate
Cathepsin K (CTSK) ELISA KitCTSK Reactivity: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Cathepsin K (CTSK) ELISA KitCTSK Reactivity: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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Comment
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FRET-based Assay Kit
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Restrictions
- For Research Use only
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Handling Advice
- Protect Components A and B from light and moisture.
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Storage
- -80 °C
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Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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- Cathepsin K (CTSK)
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Alternative Name
- Cathepsin K
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Background
- Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.
Target
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