SensoLyte® Rh110 Elastase Assay Kit

Catalog No. ABIN1882455

Quick Overview for SensoLyte® Rh110 Elastase Assay Kit (ABIN1882455)

Target: Elastase

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 /520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Component A from light and moisture.

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Component C can be stored at room temperature for convenience.

Target Details

Target: Elastase

Background: Elastases catalyze the hydrolysis of elastin, the chief constituent of elastic fibers that together with collagen determines the mechanical properties of connective tissue. Elastases have many other natural substrates these include proteoglycans, collagens and fibronectin. Implicated in many diseases such as pulmonary emphysema, cystic fibrosis, infections, inflammation and atherosclerosis, elastases are considered important drug targets.