SensoLyte® FDP Alkaline Phosphatase Assay Kit

Catalog No. ABIN1882464

Quick Overview for SensoLyte® FDP Alkaline Phosphatase Assay Kit (ABIN1882464)

Target: Fluorescein Diphosphate (FDP)

Detection Method: Fluorometric

Application: Enzyme Activity Assay (EAA)

Product Details

Brand: SensoLyte®

Characteristics: SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Store Component A at -20 °C. Store other kit components at 4 °C.

References

Zhang, Molenda, Fournelle, Murphy, Sahai: "Effects of pseudowollastonite (CaSiO3) bioceramic on in vitro activity of human mesenchymal stem cells." in: Biomaterials, Vol. 31, Issue 30, pp. 7653-65, (2010) (PubMed).

Arany, Koyota, Sugiyama: "Nerve growth factor promotes differentiation of odontoblast-like cells." in: Journal of cellular biochemistry, Vol. 106, Issue 4, pp. 539-45, (2009) (PubMed).

Target Details

Target: Fluorescein Diphosphate (FDP)

Alternative Name: FDP

Background: Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses.