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SensoLyte® Glutathione Cellular Assay Kit

EAA Fluorometric
Catalog No. ABIN1882465

Quick Overview for SensoLyte® Glutathione Cellular Assay Kit (ABIN1882465)

Target

See all Glutathione Kits
Glutathione

Detection Method

Fluorometric

Application

Enzyme Activity Assay (EAA)
  • Brand

    SensoLyte®

    Characteristics

    The SensoLyte® Glutathione Cellular Assay Kit utilizes an optimized enzymatic method for determination of the glutathione levels in cell or tissue extracts. The substrate provided in the kit can selectively react with GSH in the reaction catalyzed by glutathione S-transferase (GST) and form a fluorescent glutathione adduct. The resulting blue fluorescence can be monitored at excitation /emission= 390 nm/480 nm. The assay has a linear range of 0-100 µM of GSH. This kit does not require the usage of deproteination for biological samples.
  • Comment

    Enzymatic Activity Assay kit

    Restrictions

    For Research Use only
  • Handling Advice

    Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A from light and moisture.

    Storage

    -80 °C

    Storage Comment

    Store Components A and B at -20 °C. Store Component D at -80 °C. Components C and E can be stored at room temperature for convenience.
  • Target

    Glutathione

    Target Type

    Chemical

    Background

    Glutathione (GSH) is an important antioxidant molecule that functions as a cofactor for a variety of enzymes and is involved in various biological processes. A variation in glutathione levels is an indicator of oxidative stress.
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