HABA Biotin Quantitation Kit

Catalog No. ABIN1882419

Quick Overview for HABA Biotin Quantitation Kit (ABIN1882419)

Target: Biotin

Detection Method: Colorimetric

Application: Enzyme Activity Assay (EAA)

Product Details

Conjugate: Biotin

Characteristics: The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4'-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store Component A at -20 °C. Store all other components at 4 °C

Target Details

Target: Biotin

Target Type: Chemical