SensoLyte® 490 HIV Protease Assay Kit

Catalog No. ABIN1882480

Quick Overview for SensoLyte® 490 HIV Protease Assay Kit (ABIN1882480)

Target: HIV (Human Immunodeficiency Virus (HIV))

Reactivity: Human Immunodeficiency Virus (HIV)

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 490 HIV Protease Assay Kit uses an optimized FRET peptide substrate for a continuous measurement of HIV protease activities. This FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR with this substrate results in specific cleavage and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this kit offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Protect Components A and B from light.

Storage: -20 °C

References

Hallengärd, Haller, Petersson, Boberg, Maltais, Isaguliants, Wahren, Bråve: "Increased expression and immunogenicity of HIV-1 protease following inactivation of the enzymatic activity." in: Vaccine, Vol. 29, Issue 4, pp. 839-48, (2011) (PubMed).

Frick, Ginzburg, Lam: "A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities." in: Methods in molecular biology (Clifton, N.J.), Vol. 587, pp. 223-33, (2010) (PubMed).

Yu, Kabashima, Tang, Shibata, Kitazato, Kobayashi, Lee, Kai: "Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction." in: Analytical biochemistry, Vol. 397, Issue 2, pp. 197-201, (2010) (PubMed).

Target Details

Target: HIV (Human Immunodeficiency Virus (HIV))

Target Type: Virus, Virus

Background: HIV protease (PR) is identified as an important drug-screening target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics.