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SensoLyte® 520 MMP-10 Assay Kit

FRET Fluorometric
Catalog No. ABIN1882512

Quick Overview for SensoLyte® 520 MMP-10 Assay Kit (ABIN1882512)

Target

See all MMP10 Kits
MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))

Detection Method

Fluorometric

Application

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • Brand

    SensoLyte®

    Characteristics

    SensoLyte® 520 MMP-10 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-10, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
  • Comment

    FRET-based Assay Kit

    Restrictions

    For Research Use only
  • Handling Advice

    Protect Components A and B from light.

    Storage

    -20 °C

    Storage Comment

    Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
  • De Abrew, Thomas-Virnig, Rasmussen, Bolterstein, Schlosser, Allen-Hoffmann: "TCDD induces dermal accumulation of keratinocyte-derived matrix metalloproteinase-10 in an organotypic model of human skin." in: Toxicology and applied pharmacology, Vol. 276, Issue 3, pp. 171-8, (2014) (PubMed).

  • Target

    MMP10 (Matrix Metallopeptidase 10 (Stromelysin 2) (MMP10))

    Alternative Name

    MMP-10

    Background

    MMP-10 (stromelysin 2) cleaves extracellular matrix proteins, but is unable to cleave the triple-helical fibrillar collagens. This enzyme is involved in several pathological conditions, such as cancer, arthritis and wound healing.
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