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SensoLyte® 520 MMP-7 Assay Kit

FRET Fluorometric
Catalog No. ABIN1882524

Quick Overview for SensoLyte® 520 MMP-7 Assay Kit (ABIN1882524)

Target

See all MMP7 Kits
MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Detection Method

Fluorometric

Application

Fluorescence Resonance Energy Transfer Microscopy (FRET)
  • Brand

    SensoLyte®

    Characteristics

    SensoLyte® 520 MMP-7 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-7, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.
  • Comment

    FRET-based Assay Kit

    Restrictions

    For Research Use only
  • Handling Advice

    Protect Components A and B from light.

    Storage

    -20 °C

    Storage Comment

    Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
  • Target

    MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

    Alternative Name

    MMP-7

    Background

    MMP-7 (matrilysin, PUMP) is involved in biological events, such as tissue remodeling, cell proliferation and host defenses.
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