SensoLyte® Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity

Catalog No. ABIN1882529

Product Details

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivity: Human

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate.

Application Details

Comment:

ELISA + FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: Store all kit components, except Component B, at -20 °C. Store Component B at -80 °C. For convenience, Components D, G, H, I can be stored at RT.

References

Santos, Tewari, Lin, Kowluru: "Interrelationship between activation of matrix metalloproteinases and mitochondrial dysfunction in the development of diabetic retinopathy." in: Biochemical and biophysical research communications, Vol. 438, Issue 4, pp. 760-4, (2013) (PubMed).

Neill, Painter, Buraschi, Owens, Lisanti, Schaefer, Iozzo: "Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia inducible factor 1α, vascular endothelial growth factor A, and induction of thrombospondin-1 and TIMP3." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5492-506, (2012) (PubMed).

Moor, Vachon, Gould: "Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers." in: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 17, Issue 6, pp. 832-9, (2009) (PubMed).

Okawa, Michaylira, Kalabis, Stairs, Nakagawa, Andl, Johnstone, Klein-Szanto, El-Deiry, Cukierman, Herlyn, Rustgi et al.: "The functional interplay between EGFR overexpression, hTERT activation, and p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, and ..." in: Genes & development, Vol. 21, Issue 21, pp. 2788-803, (2007) (PubMed).

Liau, Jazag, Whang: "HMGA1 is a determinant of cellular invasiveness and in vivo metastatic potential in pancreatic adenocarcinoma." in: Cancer research, Vol. 66, Issue 24, pp. 11613-22, (2006) (PubMed).

Target Details

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: MMP-9 (MMP9 Products)