Catalog No. ABIN1882557
Quick Overview for SensoLyte® 520 Sortase A Activity Assay Kit (ABIN1882557)
Target
Sortase
Detection Method
Fluorometric
Application
Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)
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Brand
- SensoLyte®
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Characteristics
- The SensoLyte® 520 Sortase A Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of protease activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.
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Comment
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FRET-based Assay Kit
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Restrictions
- For Research Use only
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Handling Advice
- Protect Components A and B from light and moisture.
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Storage
- -80 °C
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Storage Comment
- Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Component D can be stored at room temperature for convenience
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- Sortase
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Background
- Sortases are a family of membrane-anchored transpeptidases expressed by Gram-positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.
Target
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