SensoLyte® AFC Thrombin Activity Assay Kit

Catalog No. ABIN1882568

Quick Overview for SensoLyte® AFC Thrombin Activity Assay Kit (ABIN1882568)

Target: alpha-Thrombin

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® AFC Thrombin Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate which shows high reactivity and low background. Thrombin cleaves the substrate resulting in the release of the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation/emission= 380 nm/500 nm. The assays are performed in a convenient 96-well or 384-well microplate format.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C and F can be stored at room temperature for convenience.

References

Wendeler, Pabst, Wang, Strouse, Wang, Hunter: "Process-scale purification and analytical characterization of highly gamma-carboxylated recombinant human prothrombin." in: Journal of chromatography. A, Vol. 1325, pp. 171-8, (2014) (PubMed).

Yang, Ko, Yang, Kim, Seo, Park, Choi, Seong, Kweon: "Interleukin-1 promotes coagulation, which is necessary for protective immunity in the lung against Streptococcus pneumoniae infection." in: The Journal of infectious diseases, Vol. 207, Issue 1, pp. 50-60, (2012) (PubMed).

Lim, Joy, Hill, San Brian Chia: "Novel agmatine and agmatine-like peptidomimetic inhibitors of the West Nile virus NS2B/NS3 serine protease." in: European journal of medicinal chemistry, Vol. 46, Issue 7, pp. 3130-4, (2011) (PubMed).

Target Details

Target: alpha-Thrombin

Alternative Name: Thrombin

Background: Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation. Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.